Fig 1: Western blot analysis validation of mitochondrial proteins. Liver mitochondrial expression levels of ATP5A, CytB, TFAM and Ndufa5 between the Con and HFD groups. VDAC1 was used as a loading control. (A) Images of the western blots from four biological replicates. (B) Bar graphs of the results of the western blot analysis. The values are presented as the mean ± standard deviation. *P<0.05 and **P<0.01, vs. Con group. HFD, high fat and high cholesterol diet; Con, control; ATP5A, ATP synthase a subunit; CYTB, cytochrome b; TFAM, transcription factor A, mitochondrial; Ndufa5, NADH dehydrogenase [ubiquinone] 1a subcomplex subunit 5; VDAC1, voltage-dependent anion-selective channel 1.